James Lucas

James Lucas

Developing Computational Methods for Protein Design

Sitemap

A list of all the posts and pages found on the site. For you robots out there is an XML version available for digesting as well.

Pages

Posts

Future Blog Post

less than 1 minute read

Published:

This post will show up by default. To disable scheduling of future posts, edit config.yml and set future: false.

Blog Post number 4

less than 1 minute read

Published:

This is a sample blog post. Lorem ipsum I can’t remember the rest of lorem ipsum and don’t have an internet connection right now. Testing testing testing this blog post. Blog posts are cool.

Blog Post number 3

less than 1 minute read

Published:

This is a sample blog post. Lorem ipsum I can’t remember the rest of lorem ipsum and don’t have an internet connection right now. Testing testing testing this blog post. Blog posts are cool.

Blog Post number 2

less than 1 minute read

Published:

This is a sample blog post. Lorem ipsum I can’t remember the rest of lorem ipsum and don’t have an internet connection right now. Testing testing testing this blog post. Blog posts are cool.

Blog Post number 1

less than 1 minute read

Published:

This is a sample blog post. Lorem ipsum I can’t remember the rest of lorem ipsum and don’t have an internet connection right now. Testing testing testing this blog post. Blog posts are cool.

portfolio

publications

Quantitative functional characterization of conserved molecular interactions in the active site of mannitol 2‐dehydrogenase

Published in Protein Science, 2015

Experimentally determined the contributions of active site residues toward catalysis

Recommended citation: Lucas, J.E. and Siegel, J.B. (2015), Quantitative functional characterization of conserved molecular interactions in the active site of mannitol 2‐dehydrogenase. Protein Science, 24: 936-945. doi:10.1002/pro.2669 https://onlinelibrary.wiley.com/doi/full/10.1002/pro.2669

Flex ddG: Rosetta Ensemble-Based Estimation of Changes in Protein–Protein Binding Affinity upon Mutation

Published in The Journal of Physical Chemistry B, 2018

Improving our ability to calculate protein-protein interface DDGs upon mutation

Recommended citation: Barlow KA, Ó Conchúir S, Thompson S, Suresh P, Lucas JE, Heinonen M, Kortemme T. Flex ddG: Rosetta Ensemble-Based Estimation of Changes in Protein-Protein Binding Affinity upon Mutation. J Phys Chem B. 2018 doi: 10.1021/acs.jpcb.7b11367. https://pubs.acs.org/doi/abs/10.1021/acs.jpcb.7b11367

Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs

Published in Nature Communications, 2019

Developing ligand-inducible sgRNAs to modulate Cas9 activity

Recommended citation: Kundert K, Lucas JE, Watters KE, Fellmann C, Ng AH, Heineike BM, Fitzsimmons CM, Oakes BL, Qu J, Prasad N, Rosenberg OS, Savage DF, El-Samad H, Doudna JA, Kortemme T. Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs. Nat Commun. 2019 May 9;10(1):2127. doi: 10.1038/s41467-019-09985-2. https://www.nature.com/articles/s41467-019-09985-2

talks

De Novo Ligand Binding Site Design for Improved Biosensors

Published:

I was invited to give a lightning talk on my work applying my de novo binding site design method to engineering new chemically-induced dimerization systems. I also presented a poster on my work at this event.

De Novo Ligand Binding Site Design for Improved Biosensors

Published:

I present my work developing a new method to build small molecule binding sites from scratch. I applied my method to introduce binding sites into protein-protein interfaces to create new chemically-induced dimerization systems.

teaching

Teaching experience 1

Undergraduate course, University 1, Department, 2014

This is a description of a teaching experience. You can use markdown like any other post.

Teaching experience 2

Workshop, University 1, Department, 2015

This is a description of a teaching experience. You can use markdown like any other post.